You are trying to alter the membrane potential of the neurons that you are working with in the laboratory. Which of the following would change the membrane potential of your cells?
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You are studying neurons in the lab by growing them in culture (i.e., in a petri dish outside the body). You grow them in an incubator at 37°C. With this experimental setup, you can easily change the extracellular ion concentrations by switching out the cell culture medium (i.e., the fluid that surrounds the cells).
Supreeta N.
Question 1 (20 pts) You are studying neurons in the lab by growing them in culture (in a petri dish outside the body). You grow them in an incubator at 37 degrees Celsius. With this experimental setup, you can easily change the extracellular ion concentrations by switching the cell culture medium (the fluid that surrounds the cells). Intracellular concentration Medium 1 (extracellular concentration) Medium 2 (extracellular concentration) K+ 140 mM 4.5 mM 50 mM Na+ 10 mM 135 mM 100 mM Cl- 12 mM 125 mM 120 mM In which media is the resting potential of the cell more depolarized? Assume the relative permeabilities of the ions are 100, 1, and 5 for K, Na, and Cl. Show your work.
Dominador T.
Given the following concentrations, use the Nernst equation to determine the equilibrium potentials for each ion: Inside: 130 mM Na, 125 mM K, 110 mM Cl, 0.1 mM Ca Outside: 1.0 mM Na, 0.04 mM K, 0.45 mM Cl, 0.0001 mM Ca Using the concentrations and relative permeabilities, use the Goldman-Hodgkin-Katz (G-H-K) equation to calculate the membrane potential (V) for a neuron at rest. Assume that during an action potential, the relative permeability to Na changes from 0.04 to 10 (i.e., the cell is suddenly more permeable to Na than to K). Recalculate V to determine the voltage the membrane would attain under these conditions. While recording from a neuron in the Neurobiology lab, you notice that injecting 10 nA of current into the cell causes it to depolarize by 40 mV. You determine that it reaches 63% of that voltage change in 15 msec. Given this information, determine the following: - tm (time constant) - Rm (membrane resistance)
Sri K.
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