You cut a plasmid DNA with ApaLI (G*TGCAC) and genomic DNA insert with Sfcl (C*TGCAG). You ligate the two DNAs. This ligated DNA piece can be cut with: Must incubate ApaLI and Sfcl together Neither ApaLI nor Sfcl Sfcl Either ApaLI or Sfcl ApalI
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Step 1: The restriction enzyme ApaLI recognizes the sequence G*TGCAC and cuts between the G and T. Show more…
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You have chosen to cut your DNA and plasmid with the HindIII restriction enzyme. This enzyme recognizes the sequence AAGCTT and makes a cut between the two A bases. This will generate DNA fragments with an overhang sequence of AGCT. Write the sequence of the single-stranded overhang of your plasmid DNA that would hydrogen bond with this sticky end you generated on your cut DNA.
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