Your thesis supervisor asks you to construct an artificial mini chromosome in yeast for the purpose.
Which functional elements do you need to have on such a chromosome?
After introducing your mini chromosome into cells, how would you select for cells that carry the chromosome through several generations?
Double strand breaks (DSB) are a major cause of chromosome alteration.
How can DSBs be repaired?
What happens to your mini chromosome if a double strand break is not repaired?
How can a DSB on your mini chromosome be repaired in G1 and G2, respectively?
PROBLEM 4:
You want to study mitotic loss of chromosomes as your master project.
Your thesis supervisor asks you to construct an artificial mini chromosome in yeast for the purpose.
1) Which functional elements do you need to have on such a chromosome? 2) After introducing your mini chromosome into cells, how would you select for cells that carry the chromosome through several generations?
3) Double strand breaks (DSB) are a major cause of chromosome alteration. How can DSBs be repaired? 4) What happens to your mini chromosome if a double strand break is not repaired? 5) How can a DSB on your mini chromosome be repaired in G1 and G2, respectively?