You have isolated an E. coli mutant with an aminoacyl-tRNA synthetase that causes a tRNA with the anticodon 3'-UUC-5' to be charged with asparagine at the elevated temperature of $42^{\circ} \mathrm{C}$. What effect would you expect this to have on protein synthesis in these cells at $42^{\circ} \mathrm{C}$, and why? You then isolate another mutant that suppresses the first mutation, and you trace the second mutation to a tRNA gene. What tRNA would you expect to be altered in the second mutant, and where? Predict the nature of this alteration.