Objective Biology for NEET

Rajiv Vijay

Chapter 12

Biotechnology and Its Application - all with Video Answers

Educators

Chapter Questions

Problem 1

Transgenic plants (genetically modified or GM plants) are produced by inserting desired genes in the plasmid of
(a) E. Coli
(b) Pseudomonas
(c) Bacillus subtilis
(d) Agrobacterium

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Problem 2

The applications of Biotechnology include
(A) Therapeutics
(B) Diagnostics
(C) GM crops for agriculture
(D) Processed food
(E) Bioremediation
(F) Waste treatment
(G) Energy production
(a) $\mathrm{A}, \mathrm{B}, \mathrm{C}, \mathrm{E}$ only
(b) C only
(c) B, C, D, E only
(d) All of these

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Problem 3

Golden rice is a
(a) Hybrid
(b) GM plant
(c) Transgenic plant
(d) Both (b) and (c)

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Problem 4

Which of the GMO is used by biotechnology?
(a) Microbes and fungi
(b) Plants
(c) Animals
(d) All of these

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Problem 5

Humulin is a
(a) Natural insulin
(b) Human insulin synthesized by genetically engineered $\mathrm{E}$. coli
(c) Human insulin synthesized by pancreas
(d) Chemically synthesized insulin

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Problem 6

Critical research areas of biotechnology are
(a) Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme.
(b) Creating optimal condition through engineering for a catalyst to act.
(c) Downstream processing technologies to purify the protein organic compound.
(d) All the above

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Problem 7

Salt stress, disease resistance and cold stress in plants can be introduced by
(a) Genetic engineering
(b) Tissue culture
(c) Hybridoma technology
(d) None of these

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Problem 8

Food production can be increased by
(a) Agro chemical based agriculture
(b) Organic agriculture
(c) Genetically engineered based agriculture
(d) All of these

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Problem 9

DNA fingerprinting was developed by
(a) Prof. Alec Jeffrey
(b) Arber and Smith
(c) Barbara McClintock
(d) Jacob and Monod

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Problem 10

After Green Revolution how many times does food supply has been increased?
(a) 2
(b) 3
(c) 4
(d) 5

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Problem 11

Genetic engineering is useful for
(a) Agriculture
(b) Medical research
(c) Treatment and diagnosis of diseases
(d) All of these

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Problem 12

Increased yield in Green Revolution is mainly due to
(a) Use of agrochemicals (fertilizers and pesticides)
(b) Use of improved crop varieties
(c) Use of GM crops
(d) All of these

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Problem 13

DNA fingerprinting needs
(a) Suitable restriction enzyme
(b) DNA probe
(c) Facilities for gel electrophoresis and southern blotting
(d) All the above

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Problem 14

The following are the advantages of GM crops except
(a) They reduce reliance on chemical pesticides
(b) Increase efficiency of mineral usage
(c) Enhanced nutritional value of food
(d) Post harvest losses are more

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Problem 15

Bt stands for
(a) Bacterial toxin
(b) Botulinum toxin
(c) Bacillus thuringiensis
(d) Bacillus toxin

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Problem 16

Which of the following crops are now genetically modified by Bt-toxin gene?
(A) Cotton
(B) Corn
(C) Rice
(D) Tomato
(E) Potato
(F) Soyabean
(a) A, B and C only
(b) D and E only
(c) A and $\mathrm{F}$ only
(d) All of these

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Problem 17

During electrophoresis, DNA fragments move towards the
(a) Anode
(b) Cathode
(c) Both the poles
(d) None of these

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Problem 18

Some strains of Bacillus thuringiensis produce proteins that kill certain insects like
(a) Lepidopterans
(b) Coleopterans
(c) Dipterans
(d) All of these

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Problem 19

Humulin was first marketed by
(a) Eli Lilly
(b) Sun pharma
(c) Nova Industry
(d) May and Baker

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Problem 20

Select the correct matching.
$$
\begin{array}{ll}
\hline \text { (a) Lepidopterans } & \text { Tobacco bud worm, armyworm } \\
\text { (b) Coleopterans } & \text { Beetles and bud worm } \\
\text { (c) Dipterans } & \text { Flies, mosquitoes, spiders } \\
\text { (d) Aves } & \text { Lady bird, hummingbird } \\
\hline
\end{array}
$$

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Problem 21

Antisense transcription is like carrying genes as much as
(a) On sense strand of DNA
(b) Antisense strand of DNA
(c) On genetic RNA
(d) On tRNA

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Problem 22

Bt-toxin is insecticidal because
(a) Activated toxin binds to the surface of midgut epithelial cells and creates pores that causes swelling and lysis and eventually cause death of the insect.
(b) It decreases the growth of the nervous system of the insect.
(c) It makes the insects unable to fly
(d) It kills insects by affecting its blood flow.

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Problem 23

DNA probe is used in
(a) Gel electrophoresis
(b) Western blot
(c) DNA profiling technique
(d) Interferon synthesis

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Problem 24

Bt-crops are not affected by Bt-toxin because
(a) Toxin exist as inactive protoxin.
(b) It has special antibodies against toxin.
(c) Its intracellular medium is alkaline so toxin remains inactive.
(d) All the above

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Problem 25

During DNA fingerprinting, DNA probes helps in
(a) Gel electrophoresis
(b) Southern blotting
(c) Autoradiography
(d) None of these

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Problem 26

Select the correct statement.
(a) Most Bt-toxins are insect group non-specific.
(b) Bt-toxin gene is coded with the name 'CRY'.
(c) Genes cryIAc and cryIIAb form protein that control cotton bollworms.
(d) cryIAb gene forms protein that control corn borer.

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Problem 27

Transfer of rDNA through phage is done by
(a) Diffusion
(b) Transformation
(c) Transduction
(d) Conjugation

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Problem 28

The following are all genes except
(a) cryIAc
(b) cryIAb
(c) ROP
(d) $\mathrm{SalI}$

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Problem 29

Hepatitis-B vaccine is a
(a) First generation vaccine
(b) Third generation vaccine
(c) Product of biotechnology
(d) Both (b) and (c)

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Problem 30

Protein encoded gene cryIAb controls
(a) Cotton bollworm
(b) Beetles
(c) Corn borer
(d) Flies

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Problem 31

Golden rice is enriched in
(a) Beta carotene
(b) Lysine
(c) Vitamin $\mathrm{C}$
(d) Iron

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Problem 32

Bt-toxin is obtained from
(a) Bacilli
(b) Cocci
(c) Vibrio
(d) Spirillum

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Problem 33

Engineered bacteria have successfully been used for the commercial production of
(a) Human insulin
(b) Melatonine
(c) Thyrosine
(d) Testosterone

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Problem 34

A nematode $\quad$ infects the roots of tobacco plants and causes great reduction in yield.
(a) Ancylostoma
(b) Hookworm
(c) Meloidogyne incognita
(d) Wuchereria

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Problem 35

The first transgenic crop plant was
(a) Cotton
(b) Cereals
(c) Tobacco
(d) Pea

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Problem 36

RNAi process takes place in
(a) Prokaryotes
(b) Unicellular eukaryotes only
(c) Multicellular eukaryote only
(d) All eukaryotes

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Problem 37

Which of the following cuts the DNA at a specific place?
(a) Restriction endonuclease
(b) DNA ligase
(c) Exonuclease
(d) Alkaline phosphatase

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Problem 38

Which of the following is true about RNAi process?
(a) It is a method of cellular defense.
(b) It involves silencing of a specific mRNA due to a complementary dsRNA molecule.
(c) Source of complementary RNA in this process may be infection by viruses having RNA genomes.
(d) All are true

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Problem 39

The most commonly used bacterium in plant genetic engineering is
(a) E. coli
(b) Rhizobium
(c) Klebsiella
(d) Agrobacterium

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Problem 40

Identify $\mathrm{A}$ and $\mathrm{B}$ in the diagram.
(a) A: A fully mature cotton boll, B: Destroyed by bollworms
(b) A: Destroyed by bollworms, B: Fully mature cotton boll
(c) A: Destroyed by virus, B: Immature cotton boll
(d) A: Immature normal cotton boll, B: Destroyed by virus

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Problem 41

In which of the processes is both the DNA strands transcribed?
(a) PCR
(b) DNA replication
(c) RNAi
(d) Southern blotting

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Problem 42

The source of Taq polymerase used in PCR technique is a
(a) Thermophilic fungus
(b) Mesophilic fungus
(c) Thermophilic bacteria
(d) Halophilic bacteria

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Problem 43

Which of the following vector is used to transfer nematode specific gene in host plant?
(a) Virus
(b) Rhizobium
(c) Agrobacterium
(d) Cosmid

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Problem 44

What is true about Bt-toxin?
(a) The concerned bacillus has antitoxins.
(b) The inactive protoxin gets converted into active form in the insect gut
(c) Bt-protein exists as active toxin in the bacillus.
(d) The activated toxin enters the ovary of pest to sterilize it and thus prevent its multiplication.

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Problem 45

RNAi stands for
(a) RNA infection
(b) RNA induction
(c) RNA interference
(d) RNA inhibition

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Problem 46

The abbreviation 'B' in Bt-toxin stands for
(a) Biotechnology
(b) Biotoxin
(c) Bacillus
(d) Toxin released by bacterium

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Problem 47

Which of the following is the source of complementary strand in mRNA silencing?
(a) An infection by viruses having RNA genome
(b) Mobile genetic elements (Transposons)
(c) Both (a) and (b)
(d) Proteins

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Problem 48

Some of the characteristics of Bt-cotton are
(a) Long fibre and resistance of aphids.
(b) Maximum yield, long fibre and resistance to beetle pests.
(c) High yield and production of toxin protein crystal which kills dipteran pests only.
(d) High yield and resistance to bollworms.

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Problem 49

How many recombinant therapeutics have been approved for human use all over the world?
(a) 10
(b) 20
(c) 30
(d) 90

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Problem 50

Which of the following is produced by genetically engineered bacterium?
(a) Tyrosine
(b) Insulin
(c) Glycogen
(d) $\mathrm{ADH}$

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Problem 51

How many recombinant products are presently being marketed in India?
(a) 10
(b) 20
(c) 12
(d) 30

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Problem 52

Which of the following is not used as a bioweapon?
(a) Bacillus anthracis
(b) Botulinum toxin
(c) Bacillus thuringiensis
(d) Small pox

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Problem 53

The main challenge for the production of insulin using rDNA technique is
(a) Production of A peptide
(b) Production of B peptide
(c) Getting insulin assembled into a mature form
(d) All the above

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Problem 54

Transgenic plants are produced by
(a) Inducing gene mutation
(b) Arresting spindle fibre formation
(c) Deleting sex chromosome
(d) Introducing foreign genes

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Problem 55

Peptide A and peptide $\mathrm{B}$ is linked by how many disulphide linkages between their proinsulin?
(a) 1
(b) 2
(c) 3
(d) 4

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Problem 56

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(1) Purification of humulin.
(2) Extraction of recombinant gene product from $\mathrm{E}$. coli.
(3) Culturing recombinant $\mathrm{E}$. coli in bioreactors.
(4) Introduction of recombinant plasmid into $\mathrm{E}$. coli.
(5) Synthesis of gene for human insulin artificially.
(6) Insertion of human insulin gene into plasmid.
(a) $2,1,4,3,5,6$
(b) $1,3,5,6,2,4$
(c) $5,6,4,3,2,1$
(d) $3,5,2,1,6,4$

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Problem 57

Which American company in 1983 prepared humulin?
(a) Eli Lilly
(b) Ranbaxy
(c) Sun pharma
(d) Glaxosmithkline

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Problem 58

During the process of prohormone 'proinsulin' into mature insulin synthesis
(a) C-peptide is added to proinsulin
(b) C-peptide is removed from proinsulin
(c) $\beta$ -peptide is added to proinsulin
(d) $\beta$ -peptide is removed from proinsulin

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Problem 59

Select the true statements from the following.
(1) Insulin from animal source, may develop allergy in some patients.
(2) C-peptide is not present in mature insulin.
(3) Recombinant therapeutics do not induce unwanted immunological response.
(4) Insulin can be administered orally to diabetic patients
(a) 1 and 3 only
(b) 1 and 2 only
(c) 3 and 4 only
(d) 1,2 and 3 only

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Problem 60

Cancer is generally caused due to the activation/conversion of and
or inactivation of
(a) Oncogene, tumour suppressor gene, proto-oncogene
(b) Tumour suppressor gene, oncogene, proto-oncogene
(c) Proto-oncogene, oncogene, tumour suppressor gene
(d) Oncogene, proto-oncogene, tumour suppressor gene

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Problem 61

Which of the following peptide chain is removed during maturation of pro-insulin into insulin?
(a) A-peptide
(b) B-peptide
(c) C-peptide
(d) B and C peptide

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Problem 62

The Bt-toxin is not toxic to human beings because
(a) The pro Bt-toxin inactivation requires above human body temperature.
(b) The Bt-toxin recognizes only insect specific target.
(c) The Bt-toxin formation from pro Bt-toxin requires $\mathrm{pH}$ lower than that present in the human stomach.
(d) Conversion of pro Bt-toxin to Bt-toxin takes place only in highly alkaline conditions.

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Problem 63

Which part of the diagram shows that insulin in our body is synthesized in immature form?
(a) $\mathrm{A}$ and $\mathrm{C}$
(b) $\mathrm{B}$ and $\mathrm{C}$
(c) $\mathrm{A}$ and $\mathrm{B}$
(d) None of these

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Problem 64

Why is insulin not administered orally to diabetic patients?
(a) Insulin is bitter in taste.
(b) Insulin is a peptide.
(c) Insulin will lead to sudden decrease in blood sugar if given orally.
(d) Insulin leads to peptic ulcer if given orally.

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Problem 65

The method of DNA fingerprinting involves the use of
(a) Restriction enzyme
(b) Taq polymerase
(c) Oligonucleotide primers
(d) All of these

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Problem 66

Pro-insulin contains
(a) A-peptide
(b) B-peptide
(c) C-peptide
(d) All of these

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Problem 67

What is the source of $\mathrm{T}_{i}$ plasmid which is modified and used as a cloning vector to deliver the desired genes into plant cells?
(a) Agrobacterium tumefaciens
(b) Thermophilus aquaticus
(c) Pyrococcus furiosus
(d) Aedes aegypti

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Problem 68

The first clinical gene therapy was given in
(a) 1992
(b) 1990
(c) 1995
(d) 1997

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Problem 69

The thermostable enzyme 'Taq' and 'Pfu' isolated from thermophilic bacteria are
(a) RNA polymerase
(b) DNA polymerase
(c) Restriction endonuclease
(d) DNA ligase

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Problem 70

Select the incorrect matching.
(a) $\mathrm{ADA} \rightarrow$ Adenosine Aminase
(b) ELISA $\rightarrow$ Enzyme Linked Immunosorbent Assay
(c) $\mathrm{PCR} \rightarrow$ Polymers Chain Reaction
(d) $\mathrm{PKU} \rightarrow$ Phenyl Ketonuria

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Problem 71

The term 'molecular scissor' generally refers to
(a) DNA polymerase
(b) RNA polymerase
(c) Restriction endonuclease
(d) DNA ligase

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Problem 72

ADA deficiency is due to
(a) Insertion of gene
(b) Deletion of gene
(c) Duplication of gene
(d) Translocation of gene

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Problem 73

Permanent cure for ADA deficiency is
(a) Genetically engineered lymphocyte
(b) Bone marrow transplantation
(c) Enzyme replacement therapy
(d) ADA gene introduced in early embryonic stages

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Problem 74

The first clinical gene therapy was given to a old girl.
(a) 2 year
(b) 6 year
(c) 4 year
(d) 8 year

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Problem 75

The conventional method of diagnosis involves
(a) Urine analysis
(b) ELISA
(c) $\mathrm{PCR}$
(d) rDNA technology

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Problem 76

PCR is used in the detection of
(a) HIV (AIDS)
(b) Cancer
(c) Genetic disorder
(d) All of these

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Problem 77

When a patient with defective ADA is treated, which of the following steps are performed for gene therapy?
(A) Lymphocytes are obtained from the patients.
(B) Lymphocytes are transferred to culture dishes.
(C) Lymphocytes are transected with normal ADA genes.
(D) The transected cells are returned to the patients.
(a) All the above
(b) Only $\mathrm{C}$ and $\mathrm{D}$
(c) Only D
(d) SCID cannot be treated

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Problem 78

Which of the following is a benefit to have insulin produced by biotechnology?
(a) It is just as effective and is less expensive
(b) It can be produced in large quantity
(c) It is non allergic
(d) All of these

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Problem 79

Which one of the following genes is defective in patients suffering from serve combined immunodeficiency syndrome (SCID)?
(a) RNAase
(b) $\mathrm{ADA}$
(c) Carbonic anhydrase
(d) DNAase

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Problem 80

A functional ADA cDNA can be introduced into cells of the patients receiving gene therapy by using vector constituted by
(a) E. coli
(b) Reovirus
(c) Retrovirus
(d) Agrobacterium

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Problem 81

Which of the following is used as probe?
(a) Single stranded DNA
(b) dsDNA tagged with a radioactive molecule
(c) Single stranded RNA tagged with a radioactive molecule
(d) dsRNA

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Problem 82

Which gene does not appear in photographic film in autoradiography?
(a) Housekeeping gene
(b) Structural gene
(c) Mutated gene
(d) Transcriptionally active gene

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Problem 83

Which of the following is based on antigen-antibody reaction?
(a) PCR
(b) ELISA
(c) Serum analysis
(d) Southern blotting

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Problem 84

Which of the following can be detected in ELISA?
(a) Protein antigen
(b) Glycoprotein antigen
(c) Antibodies synthesized against pathogen
(d) Any of these

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Problem 85

Over 95 per cent of all existing transgenic animals are
(a) Pig
(b) Mice
(c) Sheep
(d) Cow

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Problem 86

Transgenic organisms are used
(a) To study disease
(b) To produce biological product
(c) To test vaccine safety
(d) All of these

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Problem 87

Match the columns
\begin{tabular}{ll}
\hline Column-I & Column-II \\
\hline A. Emphysema & 1. Test to detect antigen or antibody \\
B. Rosie & 2. $\alpha-1$ antitrypsin \\
C. ELISA & 3. Protein enriched milk \\
D. ROP & 4. Codes for proteins involved in plasmid replication \\
\hline
\end{tabular}
(a) $\mathrm{A}-2, \mathrm{~B}-3, \mathrm{C}-1, \mathrm{D}-4$
(b) $\mathrm{A}-1, \mathrm{~B}-3, \mathrm{C}-4, \mathrm{D}-2$
$\mathrm{D}-4$
(c) $\mathrm{A}-1, \mathrm{~B}-2, \mathrm{C}-3$
(d) $\mathrm{A}-4, \mathrm{~B}-3, \mathrm{C}-2, \mathrm{D}-1$

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Problem 88

Transgenic animals are prepared for the following disease analysis except
(a) Cancer
(b) Cystic fibrosis and Alzheimer's
(c) Rheumatoid arthritis
(d) AIDS

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Problem 89

'Rosie' a transgenic cow known to produce a type of milk which has all the following characteristics except
(a) Protein content of $2.4 \mathrm{gm} /$ litre
(b) Has human $\alpha$ -lactalbumin
(c) More balance diet than normal milk for babies
(d) Rich in cholesterol

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Problem 90

Rosie was produced in the year
(a) 2000
(b) 1999
(c) 1997
(d) 2007

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Problem 91

GMO/transgenic animal are used in testing safety of polio vaccine before they are used on human?
(a) Transgenic sheep
(b) Transgenic cow
(c) Transgenic viruses
(d) Transgenic mice

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Problem 92

How many varieties of rice have been estimated to be present in India?
(a) 200
(b) 20,000
(c) 200,000
(d) $2,000,000$

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Problem 93

The use of bioresources by multinational companies and other organizations, without proper authorization from the countries and people concerned without compensatory payment, is called
(a) Bioethics
(b) Biopiracy
(c) Bioterror
(d) Bioweapon

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Problem 94

Which variety or rice was patented by a US company even though the highest number of varieties of this rice is found in India?
(a) Sharbati Sonora
(b) $\mathrm{Co}-667$
(c) Basmati
(d) Lerma Roja

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Problem 95

The Government of India took what step to cater to the requirement of patent terms and other emergency provisions with regard to biopiracy?
(a) Biopiracy act
(b) India Patents Bill
(c) RTI Act
(d) Negotiable Instruments Act

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Problem 96

Which Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial use?
(a) Basmati rice
(b) Turmeric
(c) Neem
(d) All of these have been targeted

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Problem 97

Golden rice is
(a) A variety of rice grown along the yellow river in China.
(b) Long stored rice having yellow colour tint.
(c) A transgenic rice having gene for $\beta$ -carotene.
(d) Wild variety of rice with yellow coloured grains.

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Problem 98

In RNAi, genes are silenced using
(a) ssDNA
(b) dsDNA
(c) dsRNA
(d) ssRNA

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Problem 99

The first clinical gene therapy was done for the treatment of
(a) AIDS
(b) Cancer
(c) Cystic fibrosis
(d) SCID (Servere Combined Immuno Deficiency resulting from the deficiency of ADA)

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Problem 100

ADA is an enzyme which is deficient in the genetic disorder SCID. What is the full form of ADA?
(a) Adenosine deoxyaminase
(b) Adenosine deaminase
(c) Aspartate deaminase
(d) Arginine dearginase

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Problem 101

Silencing of a gene could be achieved through the use of
(a) Short interfering RNA (RNAi)
(b) Antisense RNA
(c) By both
(d) None of these

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Problem 102

Identify A, B and C in the diagram.
(a) A Proinsulin, B Free C peptide, C Insulin
(b) A Free C peptide, B Proinsulin, C Insulin
(c) A Insulin, B Proinsulin, C Free C peptide
(d) A Free C peptide, B Insulin, D Proinsulin

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Problem 103

Crystals of Bt-toxin produced by some bacteria do not kill the bacteria themselves because
(a) Bacteria are resistant to the toxin
(b) Toxin is immature
(c) Toxin is inactive
(d) Bacteria encloses toxin in a special sac

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Problem 104

Assertion: ELISA is used to detect infection by pathogen, that can be detected by the presence of antigens or by detecting the antibodies synthesized against the pathogen. Reason: ELISA is based on the principle of anitgen-antibody interaction.

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Problem 105

Assertion: GEAC is genetic engineering approval committee. Reason: GEAC will make decisions regarding the validity of GM research.

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Problem 106

Assertion: IDNA therapeutic are better than similar products isolated from non-human sources. Reason: rDNA therapeutic do not induce unwanted immunological responses.

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Problem 107

Assertion: Alpha - $1-$ antitrypsin is used to treat emphysema. Reason: Transgenic mice are being used to test the safety of the polio vaccine.

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Numerade Educator

Problem 108

Assertion: PCR and ELISA are techniques that serve the purpose of easily diagnosis. Reason: For the treatment of a disease, early diagnosis is very important.

Hast Aggarwal

Numerade Educator

Problem 109

Assertion: Biopiracy is the team used to refer to the use of bio-resources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment. Reason: Bioprospecting is the process of discovery and commercialization of new products based on biological resources.

Hast Aggarwal

Numerade Educator

Problem 110

Assertion: ADA deficiency can be cured by bone marrow transplantation. Reason: ADA deficiency can be treated by enzyme replacement therapy.

Hast Aggarwal

Numerade Educator

Problem 111

Assertion: Bt toxin do not kill bacillus. Reason: Bt toxin protein exists in bacillus as inactive prototoxins.

Hast Aggarwal

Numerade Educator

Problem 112

Assertion: RNAi takes place in all eukaryotic organism as a method of cellular defence. Reason: RNAi method involve silencing of a specific mRNA due to complementary dsRNA molecule that binds to and prevent translation of the mRNA (silencing).

Hast Aggarwal

Numerade Educator

Problem 113

Assertion: Genetic modification will enhance nutritional value of food. Reason: Vitamin A enriched rice is GMO.

Hast Aggarwal

Numerade Educator

Problem 114

Assertion: GM plants are useful to us. Reason: We have introduced many useful characters in GM plants like resistance to abiotic stress, enhanced nutritional value of food.

Hast Aggarwal

Numerade Educator

Problem 115

Assertion: Bt toxin is produced by fungus Bacillus thuringienesis. Reason: Bt toxin is polysaccharide.

Hast Aggarwal

Numerade Educator

Problem 116

Assertion: serum and urine analysis is not sensitive methods of diagnosis Reason: Early detection of pathogen is not possible by these methods.

Hast Aggarwal

Numerade Educator

Problem 117

Assertion: PCR is now routinely used to detect HIV in suspected AIDS patient. Reason: Very low concentration of Virus can be detected by the amplification of the nucleic acid by PCR

Hast Aggarwal

Numerade Educator

Problem 118

. Assertion: Transgenic animals are made that carry genes which makes them more sensitive to toxic substances than non transgenic animals. Reason: Toxicity testing in such animals will allow us to obtain result in less time.

Hast Aggarwal

Numerade Educator

Problem 119

Assertion: ELISA is used to detect antigen or antibody. Reason: ELISA is a method of molecular diagnosis based on antigen- antibody reaction.

Hast Aggarwal

Numerade Educator

Problem 120

Assertion: A double stranded DNA or RNA tagged with radioactive molecule is used as probe. Reason: Because double stranded DNA or RNA is easily hybridised with single stranded DNA.

Hast Aggarwal

Numerade Educator

Problem 121

Assertion: Why oral insulin is not administered to diabetic people. Reason: Insulin is digested by our digestive enzymes.

Hast Aggarwal

Numerade Educator

Problem 122

Assertion: A functional ADA $c$ DNA is introduced in lymphocyte using retroviral vector. Reason: Disarmed retrovirus is use to deliver gene in animal cells.

Paras Aggarwal

Numerade Educator